مطالعه پارامترهای استرس اکسیداتیو و آنزیم‌های کبدی در مواجهه با نانولوله‌های کربن چنددیواره در موش‌های صحرایی

Background and Objectives: The proprietary of carbon nanotubes may cause harmful effects on the environment. In the present study, the effects of these nanoparticles on oxidative stress and liver function impairment in Wistar rats were investigated. Materials and Methods: In this experimental study, the multi-wall carbon nanotubes functionalized with carboxylic groups were injected to Wistar rats in 4 treatment groups (doses of 10 and 20 mg/kg/bw with two different blood sampling times) and control groups received physiological saline. Blood sampling was done in two stages, 24 hours and 144 hours after the last injection. Then, the activity levels of ALP (Alkaline phosphatase), ALT (Alanine aminotransferase), AST (Aspartate aminotransferase), LDH (Lactate dehydrogenase) enzymes, and malondialdehyde and plasma thiol levels were measured. Data were analyzed using one-way ANOVA and Duncan post hoc test. : In the first blood collection, LDH activity at the dose of 20 mg/kg and the amount of thiol groups at the dose of 10 mg/kg increased (p=0.006) and decreased (p=0.011) significantly than other groups, respectively. In the second blood collection, the activity levels of the ALP (p=0.020) and LDH (p=0.007) at a dose of 20 mg/kg and the activity levels of the ALT (p=0.033) at a dose of 10 mg/kg decreased significantly compared to the control group. The amount of malondialdehyde was significantly increased (p=0.023) at either doses of 10 and 20 mg/kg compared to the control group. Conclusion: Probably, carboxylic multi-wall carbon nanotubes, even at low doses, after 6 days of the last injection, causes disturbances in liver function by producing free radicals and oxidative stress. Key words: Multi-wall carbon nanotubes, Oxidative stress, Liver enzymes, Rat   Funding: This study was funded by student fees. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Islamic Azad University of Falavarjan approved the study (IR.IAUFALA.2039508230009).   How to cite this article: Nasajpour V, Noori A, Naieri H. The Study of Oxidative Stress Parameters and Liver Enzymes in Exposure to Multi-Wall Carbon Nanotubes in Rats. J Rafsanjan Univ Med Sci 2018; 17 (5): 447-460. [Farsi]